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Cloning and Expression
Enzymes
PvuII
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PvuII
PvuII from New England Biolabs
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Citations:
(7)
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New England Biolabs for
PvuII
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Description
PvuII
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
PvuII
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0151M
Quantity
25000 units
Concentration
50,000 units/ml
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
5'-CAG/CTG-3'
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (7)
(1)
Deletion of Pax1 scoliosis-associated regulatory elements leads to a female-biased tail abnormality
bioRxiv
April 18, 2023
Aki Ushiki, Rory R. Sheng, Yichi Zhang, Jingjing Zhao, Mai Nobuhara, Elizabeth Murray, Xin Ruan, Jonathan J. Rios, Carol A. Wise, Nadav Ahituv
Southern blot
analyses, genomic DNA was treated with BglIl or
PvuII
(
New England Biolabs
; R0144 or
R0151
) and fractionated by agarose gel
electrophoreses
. Following capillary transfer onto nylon
membranes
,
(2)
Increased fidelity of protein synthesis extends lifespan
Cell metabolism
November 2, 2021
Martinez-Miguel VE, Lujan C, Espie-Caullet T, Martinez-Martinez D, Moore S, Backes C, Gonzalez S, Galimov ER, Brown AEX, Halic M,
et al
.
TGCTTGTCTGGAAAAAGATT and Taq DNA polymerase (
NEB
;
M0273
) was used for
PCR
.
PvuII
restriction (
NEB
;
R0151
) was performed after
PCR
, directly in a Taq
PCR
mixture, without prior
DNA purification
. The
PCR product
(3)
High-resolution mapping of a standardized transcriptional reporter reveals positions of high and low transcription in the Escherichia coli chromosome
Cell Systems
March 1, 2019
Scott A. Scholz, Rucheng Diao, Michael B. Wolfe, Elayne M. Fivenson, Xiaoxia Nina Lin, Peter L. Freddolino
the manufacturer’s instructions. In brief, barcoded pSAS31 plasmid was digested with
PvuII
(
NEB
Cat#
R0151
) for one hour at 37°C and fragments were separated on a 0.8% agarose gel. The band corresponding to
(4)
Initiation and maintenance of pluripotency gene expression in the absence of cohesin
Genes & Development
January 1, 2015
Thais Lavagnolli, Preksha Gupta, Eva Hörmanseder, Hegias Mira-Bontenbal, Gopuraja Dharmalingam, Thomas Carroll, John B. Gurdon, Amanda G. Fisher, Matthias Merkenschlager
of
DpnII
(
R0543M
,
New England Biolabs
) overnight at 37°C for Nanog and Lefty1 and 750 U of
PvuII
(
R0151M
,
New England Biolabs
) for 2 h followed by an additional 750 U overnight at 37°C for Klf4. BAC templates
(5)
A long ncRNA links copy number variation to a polycomb/trithorax epigenetic switch in FSHD muscular dystrophy
Cell
May 11, 2012
Cabianca DS, Casa V, Bodega B, Xynos A, Ginelli E, Tanaka Y, Gabellini D.
formaldehyde for 10′ at room temperature. Digestion was performed with 600–800 U of
PvuII
(50U/ml,
NEB
,
R0151M
) at 37°C overnight with constant agitation.An appropriate amount of DNA that would amplify within the
(6)
Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
Macromolecular Bioscience
May 12, 2011
Kristopher D. Barbee, Matt Chandrangsu, Xiaohua Huang
with the wash buffer and then the reaction buffer. A solution containing 2 U · μL−1 of
PvuII
(Cat. #
R0151M
,
New England Biolabs
) in a digestion buffer (0.050 m potassium acetate, 0.020 m Tris acetate, 0.010
(7)
Fusion-Defective Gibbon Ape Leukemia Virus Vectors Can Be Rescued by Homologous but Not Heterologous Soluble Envelope Proteins
Journal of Virology
May 1, 2002
Karen B. Farrell, Yuan-Tsang Ting, Maribeth V. Eiden
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PvuII from New England Biolabs
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